| 학회 |
한국고분자학회 |
| 학술대회 |
2004년 봄 (04/09 ~ 04/10, 고려대학교) |
| 권호 |
29권 1호, p.183 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
In vitro study of large-diameter vascular grafts under pulsatile shear stress using very elastic poly(L-lactide-co-ε-caprolactone) scaffolds |
| 초록 |
Although there has been an increasing demand for prosthetic vascular graft, no viable synthetic graft was developed yet. Related problems are growth potential, poor mechanical property, biocompatibility and limited patency. In this study, very elastic, biodegradable poly(L-lactide-co-ε-caprolactone)(PLCL) was used as scaffold material. To accelerate vascular tissue in-growth and maintain its phenotype similar to native tissue, mechanical stimulation was applied during culture. Smooth muscle cells (SMCs) isolated from rabbit aortas, were cultured in 3-dimensional tubular scaffolds for 7 weeks under pulsatile shear stress. Then, endothelial cells (ECs) were seeded onto the lumen of the scaffold and cultured under a pulsatile shear stress condition for 1 week. Scanning electron microscope(SEM) images of the grown vascular tissue showed aligned SMCs parallel to pulsatile radial direction. The alignment was a typical structure of native SMCs in vascular tissue. According to western blot analysis with phenotypic markers of SMCs in a contractile state, the expression of SM-actin and SM-myosin heavy chain were up-regulated in the stretched tissues. DNA assay indicated that pulsatile radial stress increased SMCs proliferation. Histology study showed pulsatile radial stress yielded more native-like vascular tissues. |
| 저자 |
권재현1, 김수현1, 김병수2, 성원준1, 임진익1, 김영하1
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| 소속 |
1한국과학기술(연), 2한양대 |
| 키워드 |
Poly(lactide-co-ε-caprolactone); Elastic scaffold; Vascular grafts; Tissue engineering; Pulsatile radial strain
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| E-Mail |
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