| 초록 |
Tissue engineered vascular grafts have attracted a growing attention for the treatment of peripheral vascular disease (PVD). Although synthetic scaffolds using expanded PTFE and Dacronä have been successfully applied as large diameter (>5mm), high-flow vessels, there are no small-diameter vascular grafts that are viable for long-term use due to the thrombogenesis and compliance mismatch in low-flow or smaller diameter sites. We studied tissue engineered small-diameter vascular grafts using elastic, biodegradable 3D tubular scaffolds. Vascular smooth muscle cells (VSMCs) were isolated from rabbit aortas and seeded onto scaffolds. After 1week static culture, the grafts were cultured under pulsatile radial strain for 7weeks. Then, rabbit endothelial cells (ECs) were seeded onto lumen of the scaffolds and pulsatile radial strain was applied for 1weeks. A significant SMCs alignment parallel to the pulsatile radial strain was observed and the alignment was consistent with that of native vascular SM tissues. Pulsatile radial strain increased protein amount, SM Actin, SM MHC, Collagen, GAG and elastin. Also histological analysis shows that the pulsatile radial strain enhanced the alignments of SMCs and ECs, and yielded more native-like SMC and EC layer. |
