화학공학소재연구정보센터
학회 한국고분자학회
학술대회 2014년 가을 (10/06 ~ 10/08, 제주 ICC)
권호 39권 2호
발표분야 고분자합성
제목 The bioreducible poly(amido amine) polymers for in vitro gene transfection
초록 We repot new bioreducible cystamine bisacrylamide(CBA)-amine polymers as novel gene delivery carriers. Disulfied bonds in the backbone of CBA-amine polymer can be cleaved to release the genetic materials from polyplexes into cytoplasm, where higher concentration of glutathione (GSH) exists. We have synthesized and screened a library of 38 different bioreducible CBA-amine polymers by gene transfection in vitro. We have identified the lead CBA-amine polymer (CBA-106) with an extremely high transfection efficiency by inducing GFP and Luciferase expression on mouse embryonic fibroblast (MEF) cells. The stable complexation between polymer and pDNA was confirmed by gel retardation assay, AFM imaging. We also have tested the viability of transfected cells and the results were significantly higher than that of conventional gene carrier such as PEI. These results clearly indicated that CBA-amines polymers have significant potential to be used as a promising gene delviery carrier.
저자 정한샘, 이혁진
소속 이화여자대
키워드 polymer; gene delivery
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