| 초록 |
Gold nanorods (AuNRs) are often used for phototherapy with a near-infrared (NIR) laser. In the study, we utilize the cetyltrimethylammonium bromide (CTAB) immobilized gold nanorods (AuNRs) for small interfering RNA (siRNA) delivery. However CTAB is well-known toxic cationic surfactant, thus AuNRs are centrifuged to remove excess amount of CTAB and thiol-polyethylene glycol-amine (HS-PEG-NH2) is added for generating stable PEG coating on AuNRs. The PEG-coated gold nanorods (AuNRs-PEG) are consequently reacted with the succinimidyl-3-(2-pyridyldithio) propionate (SPDP) and siRNA, which is modified with –thiol (-SH). With AuNR-siRNA conjugates, we can expect to achieve effective endosomal releasing under laser stimulation and photo-triggered gene silencing can be verified in the green fluorescence protein (GFP) expressing KB cells. |
