| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 봄 (04/29 ~ 05/01, BEXCO (부산)) |
| 권호 |
19권 1호 |
| 발표분야 |
생체재료_포스터 |
| 제목 |
A Large-scale Production of Self-assembled DNA Nanostructures through Rolling Circle Amplification for siRNA Delivery. |
| 초록 |
A large-scale production of DNA nanostructures for siRNA delivery has been achieved by an isothermal enzymatic process of rolling circle amplification (RCA). Our system allowed the preparation of more than 200-fold of amplified DNA products using a ss circular DNA template. Y-shaped DNA nanostructures with sticky overhangs, which can hybridize with siRNAs, were generated through the programmed molecular self-assembly. The DNA nanostructures loaded with folate conjugated-siRNAs showed enhanced cellular uptake as well as dose-dependent gene silencing. |
| 저자 |
장보라, 홍철암, 이혁진
|
| 소속 |
이화여자대 |
| 키워드 |
siRNA; DNA nanosturcture; Rolling circle amplification (RCA)
|
| E-Mail |
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