| 초록 |
Small interfering RNA (siRNA) has recognized as a potential drug due to its target-specific gene silencing. Due to their innate physiochemical characteristics, however, the systemic delivery of naked siRNA fails to achieve therapeutically relevant gene silencing. To this end, a variety of cationic polymers and lipids have extensively studied for efficient siRNA delivery. In this study, we introduced peptides on siRNA, which can enhance the cellular uptake. In addition, we demonstrate the structure and function relation of nucleic acid nanoparticles for siRNA delivery. Holiday junction and tetrahedron nanostructures are efficiently prepared by the programmable self-assembly of DNA strands. Two distinct shapes, holiday junction and tetrahedron, has been tested for the delivery of siRNA-peptide conjugate and siRNA-peptide-holiday conjugates and their in vitro gene silencing efficacy is evaluated in detail. |
