| 학회 | 한국공업화학회 |
| 학술대회 | 2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 | 21권 1호 |
| 발표분야 | (생물공학) 4차 산업혁명을 선도하는 생물공학 신기술 |
| 제목 | Adaptive evolution and next-generation sequencing technologies for strain improvement |
| 초록 | Industrial mass production of lactic acid, which is being used as a monomer in the plastic industry, in Lactic acid bacteria (LAB) is a challenge mainly because of growth inhibition caused by the end product, lactic acid. Thus, it is important to improve acid tolerance of LAB to achieve balanced cell growth and a high titer of lactic acid. Recently, adaptive evolution has been employed as one of the strategies to improve the fitness and to induce adaptive changes in bacteria under specific growth conditions, such as acid stress. Wild type Leuconostoc mesenteroides was challenged long-term with exogenously supplied lactic acid, whose concentration was increased stepwise (for enhancement of lactic acid tolerance) during 1 year. In the course of the adaptive evolution, three mutants showing high specific growth rates and lactic acid production were isolated and characterized. Proteomic, genomic, and physiological analyses revealed that several possible factors affected acid tolerance. |
| 저자 | 이평천 |
| 소속 | 아주대 |
| 키워드 | Long-term adaptive evolution; strain improvement; lactic acid |
