화학공학소재연구정보센터
학회 한국고분자학회
학술대회 2015년 가을 (10/06 ~ 10/08, 대구컨벤션센터(EXCO))
권호 40권 2호
발표분야 의료용 고분자 부문위원회 II
제목 Mineralized Polyvinyl Alcohol/Hyaluronic Acid Hydrogel with Stiffness Gradient to Investigate Osteogenesis Behavior of Stem Cells
초록 Bone is a composite material consisting of organic matrix and inorganic mineral phase. Bone tissues have ranges of stiffness with a compressive modulus of hundreds of kPa to tenths of GPa. In this study, we prepared mineralized polyvinyl alcohol (PVA)/hyaluronic acid (HA) cylindrical hydrogel with stiffness gradient along the longitudinal direction using a simple LN2-contacted gradual freezing and thawing method and the following incubation of the hydrogel in a stimulated body fluid (SBF) solution. The morphology, mechanical properties, and differentiation behavior of bone marrow stem cells along the longitudinal direction of the prepared mineralized PVA/HA cylindrical hydrogel were investigated using the evaluations of DNA contents, RT-PCR, Western blot, and immunofluorescence stainings. PVA/HA hydrogels with stiffness gradient can be an useful tool for bone tissue engineering studies.
저자 이진호1, 오세행2, 김태호1, 유양희1
소속 1한남대, 2단국대
키워드 stiffness gradient; polyvinyl alcohol; hyaluronic acid; stem cells
E-Mail jhlee@hnu.kr