| 초록 |
Tendon injuries are a common problem in both occupational and athletic settings. However, injured tendons typically heal slowly, in particular when the tendon injury is substantial or when rupture with tendon retraction occurs. In this study, we fabricated asymmetrically porous membrane using polycaprolactone (PCL) and Pluronic F127 as a polymeric matrix for tendon regeneration. The Pluronic F127 exposed on the porous surface of the membrane could induce intermolecular hydrogen bonding with heparin. The Platelet-derived growth factor-BB (PDGF-BB) was immobilized on the PCL/Pluronic F127/haparin membranes. The amounts of heparin and growth factor immobilized on the membrane were quantified using Toluidine blue assay and ELISA kit, respectively. The in vivo animal study to evaluate the tendon regeneration potential of the PDGF-BB immobilized asymmetrically porous membranes was also investigated using a rat model (supraspinatus tendon defect). |
