화학공학소재연구정보센터
학회 한국고분자학회
학술대회 2010년 가을 (10/07 ~ 10/08, 대구 EXCO)
권호 35권 2호
발표분야 고분자 합성
제목 Self Assembly Behavior of Bio-conjugate between Nickel Complexed Nitrilotriacetic Acid-Polystyrene(Ni-NTA-PS) and Histidine-Tagged Green Fluorescent Protein(His6-GFP)
초록 Polymer-protein bio-conjugate has been widely employed in the field of biotechnology and nanotechnology. In this report, we investigated the self-assembly behavior of conjugates between histidine tagged Green Fluorescent Protein (His6-GFP) and nickel complexed nitrilotriacetic acid-polystyrene (Ni-NTA-PS). The bio-conjugate forms micelle of 60~80 nm diameters in DMF/H2O. The size of micelles decrease to 40~60 nm when GFP is released by adding of excess immidazole. The morphologies of polymer-protein bio-conjugate were confirmed by transmission electron microscopy (TEM) and dynamic light scattering (DLS).
저자 모하메드 압둘 카디르1, 한호석2, 박진홍1, 이선구1, 백현종1
소속 1부산대, 2SKC
키워드 polymer-protein bioconjugate; biotechnology; nanotechnology
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