| 학회 |
한국고분자학회 |
| 학술대회 |
2004년 봄 (04/09 ~ 04/10, 고려대학교) |
| 권호 |
29권 1호, p.199 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Preparation of poly(ethylenimine) modified by methoxy-poly(ethyleneglycol) and folic acid as a gene delivery carrier. |
| 초록 |
Recently the gene therapy using non-viral vectors draws many attentions due to the unsafety problems of viral vectors. Among the non-viral vectors, poly(ethylenimine) (PEI) is considered to be a good candidate for the gene delivery system. In this study, PEI modified by methoxy-poly(ethyleneglycol) (MPEG) and folic acid as gene carrier was synthesised to decrease a toxicity for cell and to improve in vivo targeting. First, MPEG was modified by glutaric anhydride (GA) to endow carboxylic end group, followed by the activation reaction with EDC (N-ehtyl-N'-(3-dimethylaminopropyl) carbodiimide) and NHS (N-hydroxysuccinimide). The activated carboxylic end group of PEG was reacted by the amines of PEI to give PEI modified with PEG (P1). The modified PEI (G1) was synthesized by the reaction of P1 with folate activated through EDC/NHS. The obtained P1 and G1 were characterized by 1H-NMR and FT-IR. The P1 and G1 formed the complex with DNA in various N/P charge ratio. In conclusion, we confirmed that G1 have the possibility as DNA carrier. |
| 저자 |
서동환1, 김선화2, 신병철3, 강길선2, 김문석3
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| 소속 |
1성균관대, 2전북대, 3한국화학(연) |
| 키워드 |
gene carrier; MPEG; PEI; folate
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| E-Mail |
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