| 초록 |
In cardiac microenvironments, electric pulse and cyclic mechanical strain are sequentially produced. In this study, we developed a stretchable piezoelectric substrate (SPS) that can provide PMEC to human MSCs (hMSCs) for cardiomyogenic differentiation ex vivo. Our data showed that hMSCs subjected to PMEC by SPS underwent promoted cardiac phenotype development; cell alignment and the expression of cardiac markers. The enhanced cardiac phenotype development was mediated by the up-regulation of cardiomyogenic differentiation-related autocrine factor expression and focal adhesion kinase and extracellular signal-regulated kinases signaling pathways. Thus, SPS providing electrical and mechanical regulation of stem cells may be utilized to potentiate hMSC therapies for myocardial infarction and provide a tool for study of stem cell biology. |
