| 학회 | 한국화학공학회 |
| 학술대회 | 2003년 봄 (04/25 ~ 04/26, 순천대학교) |
| 권호 | 9권 1호, p.375 |
| 발표분야 | 분리기술 |
| 제목 | Binding Study of (S)-perillyl alcohol and Human Serum Albumin using high-performance frontal analysis |
| 초록 | Quantitative investigation of drug-binding is essential in pharmacokinetic study and clinicial applications. Equilibrium dialysis and ultrafiltration followed by HPLC analysis methods have been widely used for this purpose. However, there conventional analytical methods suffer some limitations. To overcome this problem, high-performance frontal analysis(HPFA), a chromatographic method which allows simple and easy determination of unbound drug concentrations after direct sample injection has been reported. Here we present the protein analysis of (S)-perilly alcohol by HPFA. The analysis was performed on a Develosil 100-Diol-5 (10 cm x 4.6 mm I.D.) column, sodium phosphate solution (pH 7.4, ionic strength 0.17) was used as the mobile phase at a flow rate of 1ml/min. Optimum ultrawavelength was set on 205nm. A injection volume of 600 ㎕ was chosen to ensure the drug to be eluted as zonal peal with a plateau. By Scatchard analyses, it was found the binding constant (K) and binding site (n) of perillyl alcohol on HSA were: K=5.20x106[m-1], n=0.00335, respectively. |
| 저자 | 송명석, Dexian Wang, 노경호 |
| 소속 | 인하대 |
| 키워드 | HPFA; (S)-perillyl alcohol; binding paramete; HSA |
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| 원문파일 | 초록 보기 |
