| 초록 |
Due to dwindling of fossil fuel, microbial production of bio-fuel from organic byproducts has acquired significance in recent years. Ethanol has been trusted as an alternate fuel for the future. The ethanologenic pathway in Z. mobilis, like that of saccharomyces cerevisiae, consist of two essentail activities, pyruvate decarboxylase and alcohol dehydrogenase. These two activities and the enzymes of glycolysis comprise 30 to 50% of the soluble protein in Z. mobilis. By inserting Z. mobilis genes encoding pdc andadhBin E. coli. E. coli was able to ferment sugars into ethanol. This study Z. mobilis were used as the construction sources of genes and plasmids. Expression vectors were constructed by using pET-32a vectors. Ethanol productivity in E. coli strain seemed to be affected by the extent of expresstion of pdc gene along with adhB genes. By successful gene mutation we could establish a new E. coli strain which can produce ethanol efficiently. The wild-type E. coli cannot produced ethanol. So, recombination for ethanolic E. coli was investigated in this study. Also to confirm the production of ethanol, fermentation experiment of recombinant E. coli was performed in aerobic conditions. |
