| 초록 |
The extracellular matrix (ECM) provides instructive cues for cell attachment, proliferation, differentiation, and most importantly, it preserves tissue native environment. The use of decellularized ECM (dECM) in regenerative-medicine approaches has been rapidly expanding, especially in cartilage regeneration. For cartilage regeneration, it needs to develop a bio-ink with biocompatibility, biodegradability, and good printability. Cellulose nanofiber (CNF) has gained increased interest for biomedical applications because of its unique properties, including sustainability, biodegradability, biocompatibility, high surface area, and good strength properties. Sodium alginate (SA) also has good biocompatibility, cross-linking properties, and it can form a gel under mild conditions. The study's objective was to prepare a hydrogel type 3D printable bio-ink by directly mixing dECM, TEMPO-oxidized cellulose nanofiber (TOCN), and alginate to fabricate a functional biomolecule-containing scaffold. A calcium chloride solution was sprayed during the printing process to partially cross-link alginate and increase the printed gel's dimensional stability. At the end of the printing process, the prepared scaffolds were immediately cross-linked using calcium chloride to provide the hydrogel's rigidity and give it long-term stability. TEMPO-Oxidized CNF was used as support for cross-linked alginate through the new TEMPO-induced carboxyl groups' participation in constructing the cross-linked network. To better understand the mechanisms governing the manufacturing of scaffolds by 3D printing, the rheological behaviour and cell biocompatibility were investigated. |
