| 초록 |
Polymeric micelles have emerged as highly integrated carriers for theragnosis. However, micelles often tend to release drugs rapidly due to a large volume dilution upon entering the bloodstream, thereby resulting in undesirable toxicity and low theragnostic efficacy. In this study, we developed in situ cross-linkable micelle (CM) via enzyme-mediated reaction to improve stability of the micelles carrying SPIO and drugs. The DLS data showed that there was no significant time-dependent change in the size of the CM (~100 nm) but increased hydrodynamic diameters of the non cross-linked micelles (NCM) was observed with increasing time. The in vitro cell viability result using NIH3T3 exhibited that the CM is relatively non-toxic even at the highest concentration of 1 mg/mL after 48 h culture, while both NCM significantly reduced cell viability up to 50%. The obtained results demonstrate that the CM prepared by enzyme-mediated reaction can be useful to deliver SPIO and drugs for theragnosis. |
