| 초록 |
Adenovirus encoding an exogenous green fluorescent protein (GFP) was physically immobilized with cationic graft or block polymers via ionic interactions. Poly-L-lysine (PLL) blocked or grafted with poly(ethylene glycol) (PEG) coated on the surface of adenovirus exhibited enhanced gene expression compared to non-modified poly-L-lysine into the cancer cell line as well as primary cell. The degree of PEG graft per PLL influenced the stability of adenovirus, in vitro transfection, and cytotoxicity. Furthermore, type of copolymer, PEG-PLL block copolymer or graft copolymer, also affected the extent of viral uptake into cells. Thus, the study suggests that AD coupled with PLL-b-PEG could be developed as gene delivery carriers for an efficient gene transfer and low cytotoxicity. |
