| 학회 |
한국화학공학회 |
| 학술대회 |
2015년 가을 (10/21 ~ 10/23, 일산 KINTEX) |
| 권호 |
21권 2호, p.1746 |
| 발표분야 |
생물화공 |
| 제목 |
Engineering E. coli for the production of 3-aminopropionic acid |
| 초록 |
We report metabolic engineering of Escherichia coli for the production of 3-aminopropionic acid (3-AP). A fumarate-producing E. coli strain was modified by chromosomal overexpression of aspA (aspartase), enabling conversion of fumarate to aspartate. Aspartate was then converted to 3-AP by expression of the C. glutamicum panD gene (L-aspartate-α-decarboxylase). Additional overexpression of the aspA and phosphoenolpyruvate carboxylase (ppc) genes allowed production of 3.49 g/L 3-AP. Further optimization of the ppc expression, combined with chromosomal overexpression of acs, resulted in production of 32.3 g/L 3-AP in 39 h in fed-batch. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF) of Korea (NRF-2012-C1AAA001-2012M1A2A2026556). MG also received support from the Swedish research council Formas]. |
| 저자 |
이상엽1, Chan Woo Song2, Martin Gustafsson3
|
| 소속 |
1MBEL, 2Department of Chemical and Biomolecular Engineering (BK21 program), 3KAIST / BioInformatics Research Center |
| 키워드 |
Escherichia coli; Metabolic engineering; 3-Aminopropionic acid; Fumaric acid
|
| E-Mail |
|
| 원문파일 |
초록 보기 |
