| 학회 |
한국화학공학회 |
| 학술대회 |
2015년 가을 (10/21 ~ 10/23, 일산 KINTEX) |
| 권호 |
21권 2호, p.1750 |
| 발표분야 |
생물화공 |
| 제목 |
Efficient fumaric acid production using E. coli with a re-designed TCA cycle |
| 초록 |
In this work, E. coli was engineered to produce fumaric acid. The iclR gene was deleted to redirect the carbon flux through the glyoxylate shunt. This, together with the deletion of fumA, fumB and fumC, resulted in production of fumaric acid to 1.45 g/L. Plasmid-based overexpression of the ppc gene further increased the titer to 4.09 g/L. Next, arcA and ptsG were deleted to reinforce the oxidative TCA cycle, and aspA was deleted to block the conversion of fumaric acid to L-aspartate. Finally, galP was overexpressed to improve the glucose uptake. Fed-batch culture of the final strain produced a fumaric acid titer of 28.2 g/L. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF) of Korea (NRF-2012-C1AAA001-2012M1A2A2026556). MG was additionally supported by the Swedish research council Formas]. |
| 저자 |
이상엽1, Jae Won Jang2, Sol Choi3, Dong In Kim4, Chan Woo Song5, Martin Gustafsson1
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| 소속 |
1MBEL, 2Department of Chemical and Biomolecular Engineering (BK21 program), 3KAIST / BioInformatics Research Center, 4BioProcess Engineering Research Center, 5KAIST |
| 키워드 |
Metabolic engineering; Escherichia coli; Fumaric acid
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| E-Mail |
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| 원문파일 |
초록 보기 |
