| 학회 |
한국화학공학회 |
| 학술대회 |
2015년 가을 (10/21 ~ 10/23, 일산 KINTEX) |
| 권호 |
21권 2호, p.1754 |
| 발표분야 |
생물화공 |
| 제목 |
Production of L-malic acid using a metabolically engineered Mannheimia succiniciproducens strain |
| 초록 |
L-Malic acid (MA) is a commonly used chemical. The Gram-negative bacterium Mannheimia succiniciproducens has a strong anaplerotic pathway, suggesting that it has good potential for malic acid production. Thus, we aimed to create an efficient malic acid production strain, starting from a succinic acid producing M. succiniproducens previously developed in our lab. By deletion of the fumC gene encoding fumarase, the conversion of malic acid to fumarate was blocked, resulting in accumulation of malic acid as the main product. These results provide useful information for the rational metabolic engineering to improve MA production in strains with strong anaplerotic pathways. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF) of Korea (NRF-2012-C1AAA001-2012M1A2A2026556). MG was additionally supported by the Swedish research council Formas]. |
| 저자 |
이상엽1, Sol Choi2, Martin Gustafsson3
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| 소속 |
1MBEL, 2Department of Chemical and Biomolecular Engineering (BK21 program), 3KAIST / BioInformatics Research Center |
| 키워드 |
L-malic acid; Metabolic engineering; Mannheimia succiniproducens
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| E-Mail |
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| 원문파일 |
초록 보기 |
