| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 가을 (10/06 ~ 10/07, 김대중 컨벤션센터) |
| 권호 |
36권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Targeted delivery of Cell PenetratingMicroRNAgene carrier for Breast Cancer therapy |
| 초록 |
Drug delivery system with cell-penetrating pepetide (CPP) has gained greater attention in biomedical field. CPP facilitates intracellular uptake of target materials such as protein, gene, or macromolecules. Development of therapeutic molecule was greatly thwarted by poor uptake efficiency and low cellular trafficking, which might be overcome by chemical conjugation with CPP. In this study, we conjugated newly identified cellular penetrating peptide, which was found to be highly specific to breast cancer and conjugated to cationic gene carrier for achieving enhanced intracellular uptake. MicroRNA to be delivered with CPP-conjugated gene carrier for the treatment of breast cancer. The complex formation with plasmid DNA was checked by gel electrophoresis. Particle size and the surface charge were determined by DLS and zeta potential. The transfection efficiency and cytotoxicity of the carrier has been studied with various breast cancer cell lines. |
| 저자 |
이화정1, 남궁란2, 김원종2, 김재일3, 박인규1
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| 소속 |
1전남대, 2포항공과대, 3광주과학기술원 |
| 키워드 |
Breast cancer; MicroRNA; Gene carrier
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| E-Mail |
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