| 초록 |
Rheumatoid arthritis (RA) is an autoimmune disease that causes constant inflammation on synovial tissues by excessive immune response. Although immune modulating drug, methotrexate(MTX), is used as therapeutic agent, it has no site specificity and causes numerous side effects. To alleviate adverse effects, we prepared MTX loaded hyaluronic acid (HA) and 5β cholanic acid (CHOL) conjugate linked with acid-labile linker (MTX@HA-pH-CHOL). Since HA has specific binding affinity with CD44 receptor, the MTX@HA-pH-CHOL could be delivered to lesion area selectively. Furthermore, in the mild acidic RA region, MTX is released through the cleavage of pH-sensitive linker. In vitro drug release test demonstrated that HA-pH-CHOL shows pH sensitive degradation behavior. Moreover, in vitro cellular uptake experiment, we confirmed HA improves the internalization of MTA@HYA-pH-CHOL to RAW 264.7 cells. Overall, the HA-based MTX contained agents with pH-sensitivity could be applied to therapeutics of RA |
