| 초록 |
We investigated DNA fragments separations by capillary electrophoresis using coating materials that differ in linear alkyl-group size and the bulkiness of the substituent group. By the introduction of Si-O-Si linkages, low optical loss materials suitable for device-quality applications have been obtained. However, they are unstable as the pH increases, which is the favorable condition for high resolution. In this study, several coating materials, such as chlorotrimethylsilane, n-butyldimethylchlorosilane, chlorodimethyloctylsilane, t-butyldimethylchlorosilane, and triethylchlorosilane, have been evaluated experimentally to produce a coating with high resistance to the separation conditions. These coatings were tested for how much they reduced the electroosmotic flow and how much they were reproducible. They were also tested for resolution, efficiencies, and stability.
|
