초록 |
Herein, we propose a novel approach to further facilitate the internal starch synthesis of C. reinhardtii through the operation of carbon-concentrating mechanism along with a two-stage process based on sulfur deprivation, thereby resulting in enhanced anaerobic capacity during PSII-dependent H2 photoproduction phase. When CCM-induced cells were exposed to high levels of CO2 with S deprivation internal levels of starch was significantly elevated by retaining a functional CCM with the boosted photosynthetic activity during 24 of S deprivation. As a result, during H2 production phase (III) at irradiance of 50 μE m–2 s–1, the concentrations of starch and hydrogen in CCM-induced cells were remarkably enhanced by 61.4% and 218.9% compared to that of CCM-uninduced cells, respectively. The treatment of low-CO2-driven CCM induction prior to S deprivation is a low-cost and energy strategy that improves the autotrophic H2 photoproduction by microalgae. |