| 초록 |
Microbial natural products have long been one of the major sources of clinically important drugs. Culture-based extract screening in combination with bioactivity-guided isolation has been the major platform for the discovery of biologically active compounds from microbial sources for the past century. However, recent (meta)genome sequencings have revealed that most of biosynthetic gene clusters remain silent in standard culture conditions, and yet there are a significant number of uncultured bacteria present in nature. eDNA are therefore a large reservoir of previously uncharacterized biosynthetic gene clusters. Metagenomics provides a way to access this hidden biosynthetic diversity present in nature with the aid of next generation sequencing, bioinformatics and synthetic biology tools. In this seminar, I will present some examples of how new biologically active small molecules are discovered using metagenomics in combination with synthetic biology tools. |
