| 초록 |
The unit cost for the production of algal biofuel needs to be reduced in order to be a substitute for fossil fuel. Here, we developed a PDMS-based multiplex microfluidic system with eight chambers and micropillar arrays to expedite multiple steps for lipid sample preparation from different microalgal strains. We could rapidly and efficiently perform sequential operations from cell culture to lipid extraction of eight different microalgal strains simultaneously on a single device without harvesting and purification steps, which are labor- and energy-intensive, by the simple injection of medium and solvent into the central inlet due to the integrated micropillar arrays connecting the chambers and central inlet. The lipid extraction efficiency using this system was comparable (94.5–102.6%) to the conventional Bligh–Dyer method. These results demonstrate that our multiplex microfluidic approach can provide an efficient analytical tool for the rapid analysis of strain performances. |
