| 초록 |
Pancreatic islets transplantation is a promising method of cell therapy that can be used to treat type 1 diabetes mellitus. The aim of this study was evaluation of combinatorial therapy using PEGylation and immunosuppressant in the transplantation of pancreatic islet allograft and xenograft. Islets were isolated from male Sprague-Dawley (SD) rats and then PEGylated with mPEG-SPA, which can rapidly react with amine groups of collagen matrix that composes the outer surface of isolated islets. On the left kidney of STZ-induced diabetic recipient C57BL/6 mice and F344 rats, the unmodified islets or PEGylated islets was transplanted. During the injection of 3 mg/kg/day of CsA, MST of unmodified islet allografts in rats was 7 days, whereas MST of PEGylated islet allografts was more than 100 days. However, During the injection of 0.5 mg/kg/day of Tacrolimus, MST of unmodified islet xenografts in mice was 13 days, whereas MST of PEGylated islet xenografts was 21 days. |
