| 초록 |
Extracellular matrix (ECM) provides cells with structural integrity and biofunctional contacting surface for tissue formation. In order to mimic structural and physiological properties of ECM, we developed nanofibers blended with poly(l-lactic acid-co-ε-carprolactone) (PLCL) and polyaniline (PANi) by using electrospinning technology. We then cultured skeletal myoblasts (C2C12) on the nanofibers, which had various blending ratios of PANi to PLCL and investigated their effects on proliferation and degrees of myogenic differentiation by means of DNA assay, immunofluorescence staining, western blotting, and Real-time RT PCR. Our results indicate that there was no significant difference in cell proliferation among all groups, however, depending on the blending ratios of PANi, the cells on PLCL/PANi nanofibers showed more differentiated phenotype, resulting in the formation of assembled myotube, rather than those on the PLCL nanofibers. |
