| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 가을 (10/11 ~ 10/12, 일산킨텍스) |
| 권호 |
32권 2호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Preparation of PEG-coated lipid nanoparticles containing amphotericin B and their in vitro and in vivo characteristics |
| 초록 |
Lipid nanoparticles (LNP) are drug delivery carriers due to their solubilizing capability and toxicity reduction of poorly water-soluble drugs. Clinical usage of Amphotericin B (AmB), a polyene antibiotic, is limited due to its nephrotoxicity. In this study AmB was entrapped to LNP composed of phospholipid and cholesterol. PEG-coated LNP containing AmB were prepared by incorporating a DSPE-mPEG into lipid composition of LNP. Mean particle size of AmB-entrapped LNP was about 65±7 nm. Surface charge of the AmB-entrapped LNP was negative showing -54.2±4 mV of zeta potential value. AmB entrapment efficiency to LNP reached up to 85±4%. AmB-entrapped LNP were prepared by freeze-drying method. Mean particle size of the AmB-entrapped LNPs was determined before and after freeze-drying to evaluate the LNP stability. In order to investigate effects of PEG on in vitro toxicity of AmB-loaded LNP, cytotoxicity against human kidney cells and hemolysis of red blood cells were evaluated. |
| 저자 |
임덕휘, 정석현, 성하수, 신병철
|
| 소속 |
한국화학(연) |
| 키워드 |
Lipid nanoparticles; LNP; amphotericin B; PEG-coated
|
| E-Mail |
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