| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 가을 (10/11 ~ 10/12, 일산킨텍스) |
| 권호 |
32권 2호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Improving Gene Silencing Efficiency of PEGylated siRNA Complexed with Temperature-Sensitive Pluronic/Poly(ethylenimine) Nanocapsules: Cold Shock Induced Endosome Breaking Effect |
| 초록 |
Pluronic/PEI nanocapsules (NCs) containing a hollow interior structure were synthesized by cross-linking between pre-activated Pluronic F-127 and poly(ethylenimine) at an oil-in-water interface during a modified emulsification/solvent evaporation process. These NCs exhibited reversible swelling/deswelling volume transition in response to temperature. The size of NCs measured by DLS was about 160 nm at 37 °C and about 400 nm at 15 °C. This transition was caused by temperature-dependent hydrophobic interaction of Pluronic grafted to NC shell layer. We previously proved that Pluronic/PEI NCs could be utilized as an endosome breaking agent by lowering the temperature below a phase transition temperature, which triggers rapid volume expansion upon cooling. PEGylated VEGF or GFP siRNA (siRNA-PEG) was complexed with Pluronic/PEI NCs, delivered into the cells, and the gene silencing effect was comparatively examined with and without the cold shock treatment to verify the hypothesis. |
| 저자 |
이수현, 박태관, 이규리
|
| 소속 |
한국과학기술원 |
| 키워드 |
siRNA; temperature-sensitive; nanocapsules; endosome breaking; gene delivery
|
| E-Mail |
|
