| 초록 |
With high specificity and efficiency in target gene inhibition, small interfering RNAs are promising tool for genomic therapeutics. However several cationic carriers, which have already shown their good transfection capacity in DNA delivery, were not competent in siRNA delivery because of low charge density and small size of siRNA compared to large plasmid DNA. To overcome insufficient charge density and form more compact and stable complex with cationic carrier, biodegradable and biocompatible PLGA was conjugated to 3’ end of siRNA by a disulfide bond. In an aqueous environment, siRNA-PLGA conjugates were self-assembled to form a micellar structure because of their amphiphilic property. The hydrophobic PLGA part contributed to increase the siRNA charge density and improve the complex stability after formation of polyelectrolye complexes with cationic carriers. As a result, the enhanced target gene inhibition was demonstrated with siRNA-PLGA using linear PEI as a cationic carrier. |
