| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 가을 (10/08 ~ 10/09, 광주과학기술원 오룡관) |
| 권호 |
34권 2호 |
| 발표분야 |
약물 및 유전자 전달용 고분자(의료용 고분자 부문위원회) |
| 제목 |
Target Specific Intracellular Delivery of anti-VEGF siRNA Using Hyaluronic Acid Reducible PEI [HA-(PEI-SS)] Conjugate |
| 초록 |
Target specific intracellular siRNA delivery systems have been developed using hyaluronic acid (HA) reducible polyethyleneimine (PEI) conjugate. To alleviate the inherent cytotoxicity and low serum stability of high molecular weight (MW) PEI, relatively non-toxic low MW PEI was crosslinked with cystamine bisacrylamide (CBA) to synthesize reducible PEI-SS in the cytosol. Then, PEI-SS was conjugated with HA in the forms of HA-g-(PEI-SS) and HA-b-(PEI-SS) to enhance serum stability and facilitate target specific delivery of siRNA by receptor. The HA-(PEI-SS) conjugates could make a complex with siRNA showing negative surface charge. The resulting HA-(PEI-SS)/siRNA complex demonstrated an excellent gene silencing efficiency. Moreover, the cytotoxicity of HA-(PEI-SS) was negligible likely due to the degradation of PEI-SS. In vivo gene silencing tests using anti-VEGF siRNA will be carried out for the treatment of various angiogenic diseases including cancer and asthma, and liver cancer. |
| 저자 |
박기태, 이민영, 한세광
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| 소속 |
포항공과대 |
| 키워드 |
Hyaluronic acid; Polyethyleneimine; siRNA; Target delivery
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| E-Mail |
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