| 학회 |
한국화학공학회 |
| 학술대회 |
2011년 봄 (04/27 ~ 04/29, 창원컨벤션센터) |
| 권호 |
17권 1호, p.372 |
| 발표분야 |
생물화공 |
| 제목 |
Highly Efficient Electrochemical Biosensing Platform by Employing Conductive Nanocomposite Entrapping Magnetic Nanoparticles and Oxidase in Mesoporous Carbon Foam |
| 초록 |
A conductive multi-catalyst system consisting of Fe3O4 magnetic nanoparticles (MNPs) and oxidative enzyme co-entrapped in the pores of mesoporous carbon is developed as an efficient and robust electrochemical biosensing platform. The construction of the nanocomposite begins with the incorporation of MNPs by impregnating Fe(NO3)3 on a wall of mesoporous carbon followed by heat treatment under an inert atmosphere, which results in the formation of magnetic mesoporous carbon (MMC). Glucose oxidase (GOx) is subsequently immobilized in the remaining pore spaces of the MMC by using glutaraldehyde crosslinking to prevent enzyme leaching from the matrix. H2O2 generated by the catalytic action of GOx in proportion to the amount of target glucose is subsequently reduced into H2O by the peroxidase mimetic activity of MNPs with the generated cathodic current, which can be detected through the conductive carbon matrix. |
| 저자 |
김문일1, 예영진2, 원병연1, 신수정1, 이진우2, 박현규1
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| 소속 |
1KAIST, 2POSTECH |
| 키워드 |
nanocomposite; electrochemical biosensor; mesoporous carbon
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| E-Mail |
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| 원문파일 |
초록 보기 |
