| 학회 |
한국화학공학회 |
| 학술대회 |
2012년 봄 (04/25 ~ 04/27, 제주 ICC) |
| 권호 |
18권 1호, p.396 |
| 발표분야 |
생물화공 |
| 제목 |
Quantum dots induced by ultraviolet rays supply electrons for cytochrome P450 reaction |
| 초록 |
We elucidated the influence of electron formation from ultraviolet (UV)-induced quantum dots (Qdots) on the cytochrome P450 (CYP2B4) reaction. Electron formation was effectively triggered by inducing Qdots with (UV) irradiation at 365 nm. The analytical potential of the Qdots-based electron-transfer system was demonstrated by examining two different types of cofactors, i.e., chemical (NADPH) and nanoparticle (Qdots). The Qdots-based method gave promising results in comparison with the NADPH-dependent system. A Qdots-based electron supply to a CYP2B4 and 7-pentoxyresorufin incubation mixture produced a resorufin metabolite. We determined the kinetic rates of the cytochrome P450 reactions induced by photoactivated Qdots. The process comprised UV-induced electron formation by photoactivated Qdots and subsequent enzyme reactions mediated by the resultant electron supply. |
| 저자 |
노창현 |
| 소속 |
한국원자력(연) |
| 키워드 |
Nanoparticle; P450; UV; electron
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| E-Mail |
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| 원문파일 |
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