화학공학소재연구정보센터
학회 한국공업화학회
학술대회 2012년 가을 (10/31 ~ 11/02, 대전컨벤션센터)
권호 16권 2호
발표분야 포스터-생물공학
제목 Increase of Bioactive Compounds by UV-induced Mutants of Microalgae
초록 Arthrospira platensis (A. platensis) is the main microalga produced commercially, whose cultivation is particularly attractive for several reasons. It contains high levels of proteins, lipids and vitamins. Isolation of mutants by chemical or physical mutagen is a clue for strain improvement. In this study, effects of ultraviolet-B (UV-B) radiation on A. platensis were investigated and several mutants by UV-B were obtained. The A. platensis were exposed to UV-B (15 Watt, 254 nm). Resulting mutants were designated UM1, UM3, UM5 and UM10, respectively. Colonies were isolated and further cultured in liquid medium. After 12 days after cultivation, biomass, protein, chlorophyll, carotenoid and lipid content were measured. Especially, lipid content of UM5 mutant was considerably increased 8∼11-fold compared to wild type. Moreover, carotenoid content and antioxidant enzyme activity was increased. Development of microalgae strain producing high lipid content will be useful in the fuel industry.
저자 박현진, 김영화, 이재화
소속 신라대
키워드 microalgae; Arthrospira platensis; UV-B; mutant
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