| 학회 | 한국화학공학회 |
| 학술대회 | 2008년 봄 (04/23 ~ 04/25, 제주ICC) |
| 권호 | 14권 1호, p.535 |
| 발표분야 | 생물화공 |
| 제목 | Clinically important pathogen detection by microarray validated with various clinical isolates |
| 초록 | A rapid and precise diagnosis of bacterial infection is important for proper therapy to patients. However, bacterial detection by the traditional method containing cultivation is time consuming and not sensitive enough. DNA microarray-based assay provides more reliable, efficient, reproducible, precise and high throughput detection in a short assay time than current techniques. In this study, we suggest diagnostic microarray for 39 pathogenic bacteria, which selected based on high prevalence and/or difficulty of cultivation. Based on the 23S ribosomal DNA (rDNA) and 16S-23S rDNA intergenic spacer region sequence analysis, the bacteria- and bacterial species-specific probes were designed by considering relations between the probe properties and hybridization efficiency. The probes were microarrayed on the slide glass and the usefulness of these selected probes was confirmed by hybridizing with DNAs from reference bacteria and 515 clinical isolates. Our diagnostic microarray for pathogen showed 97.7% sensitivity, 100% specificity and positive predictive value. [This work was supported by Medigenes Co., Ltd.] |
| 저자 | 유승민1, 장경희2, 유내춘2, 금기창2, 유원민2, 김준명2, 이상엽1 |
| 소속 | 1KAIST 생명화학공학과, 2연세대 |
| 키워드 | Microarray; Pathogen; Detection; Probe |
| 원문파일 | 초록 보기 |
