| 초록 |
We demonstrate a facile method to fabricate a recyclable cell-alignment scaffold using nanogrooves based on sublimable liquid crystal (LC) material. Randomly and uniaxially arranged smectic LC structures treat with osmium tetroxide (OsO4), the nanogroove can serve as a scaffold to efficiently induce directed cell growth without causing cytotoxicity, and can be used repeatedly. Together, various cell types are applied to the nanogroove, proving the scaffold's broad applicability. Depending on the nanotopography of the LC structures, cells have different morphologies and gene expression levels compared to cells on glass substrates, according to confocal and qPCR. Furthermore, cell sheets can be formed which consist of oriented cells, and can be repeatedly transferred to other substrates while maintaining its organization. We believe that our cell-aligning scaffold may give the way for the soft material field to bioengineering, as well as applications for regenerative medicine. |
