| 학회 | 한국공업화학회 |
| 학술대회 | 2016년 봄 (05/02 ~ 05/04, 여수 엑스포 컨벤션) |
| 권호 | 20권 1호 |
| 발표분야 | 생물공학_포스터 |
| 제목 | MALDI-MS based quantitative analysis for ketone containing bacterial quorum sensing molecules |
| 초록 | Gram-negative bacteria secrete N-acyl homoserine lactones (AHLs) as quorum sensing modulators. Although various analytical techniques have been developed for detection and quantitation of AHLs used cell-to-cell communication, only a few methods have been applied to AHL identification. Here, We developed a highly sensitive and reliable MALDI-MS based 3-oxo AHL quantitation method by introducing Girard’s reagent T (GT) to produce a permanent cationic charge state [M]+ at the ketone group of AHLs. After extracting AHLs from the supernatant of bacterial cultures using ethyl acetate, the extracts were subsequently derivatized with GT. The chemical derivatization of 3-oxo AHLs dramatically enhanced sensitivity by lowering the limit of detection and limit of quantitation and allowed more accurate quantitative analysis. This method may be applied for developing high-throughput and sensitive detection methods of quorum sensing signal molecules in biofilm-related clinical applications. |
| 저자 | 김경진, 박한규, 황철환, 김윤곤 |
| 소속 | 숭실대 |
| 키워드 | N-acyl homoserine lactone; Girard's reagen T |
