| 초록 |
A contact lens is ideal to monitor glucose levels in tear. We designed a contact lens-based biosensor comprised of glucose oxidase (GOX) and cerium oxide nanoparticle (CNP) to detect glucose levels in tear. GOX catalyzes the oxidation of glucose to H2O2 and gluconolactone. Then, CNP catalyzes the reduction of H2O2. At this moment, Ce3+ being colorless shift to Ce4+ state that is shown yellow color. Glucose levels can be determined by analyzing the change of color. B value of RGB color is used to determine glucose levels, being shown the correlation with glucose concentration. To confirm the synthesized CNP structure, we perform the XPS, XRD, HR-TEM. GOX is immobilized on a modified-CNP using PEG spacer; it is CNP-PEG-GOX. The formation of CNP-PEG-GOX is determine via quantitative analysis of GOX. The contact lens sensor maintains its mechanical properties compared with HEMA contact lens. and has correlation with glucose levels in buffer and artificial tear. |
