| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 봄 (04/07 ~ 04/08, 대전컨벤션센터) |
| 권호 |
36권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Synthesis and Charecterisation of Functional Nanoparticles for MRI guided gene delivery |
| 초록 |
SPION-based diagnostic properties with accompanying therapeutics such as drugs or genes has been explored to improve their therapeutic efficacy. The positively charged SPION-loaded polymersome tethering PEG as a hydrophilic corona layer was prepared to deliver therapeutic genes to target sites, which was monitored by MRI concomitantly. SPION-loaded polymersome with plasmid DNA complex was checked by gel retardation assay . Hydrodynamic particle size was between 110 and 160nm, positive surface charge confirmed by zeta potential. The TGA studies confirmed that spion embedded polymerosome shows more thermal stability. The transfection efficiency in vitro was tested by luciferase assay.The detectability of SPION in transfected cells by MRI was also checked. In vivo accumulation of polymerosome loaded spion in the tumor region was confirmed by MRI imaging and histo chemical analysis .Result indicates that the SPION-embedded supra-assembly can be applied to MR imaging guided gene therapy. |
| 저자 |
이상준1, MUTHUNARAYANAN MUTHIAH1, 이현진2, 문명주3, Hieu1, 이화정1, 차혜련1, 정용연1, 박인규1
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| 소속 |
1전남대, 2KAIST, 3화순전남대병원 |
| 키워드 |
Spion; polymersome; gene delivery; MRI
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| E-Mail |
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