| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 봄 (04/07 ~ 04/08, 대전컨벤션센터) |
| 권호 |
36권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
siRNA Hydrogel Microparticles for Gene Silencing |
| 초록 |
We demonstrated a novel strategy to prepare biologically active siRNA-based hydrogel structures through self-assembly process between complementary ss sense and antisense siRNA species using Y-shaped siRNA as crosslinkers. The siRNA-based microgels with substantially increased charge density were drastically collapsed to form compact nano-sized complexes upon interacting with a less charged cationic polymer carrier, and the complexes exhibited superb cellular uptake and gene silencing activities. In the near future, nano-sized siRNA hydrogel particles could also be fabricated by using a microfluidic device that allows controlled hybridization between the two complementary ss sense and antisense siRNA species in a small mixing volume. Such functionally active siRNA-based nanostructures hold great potentials as a new class of nucleic acid platform materials for therapeutic applications. |
| 저자 |
홍철암, 박태관
|
| 소속 |
KAIST |
| 키워드 |
siRNA; hydrogel; gene silencing
|
| E-Mail |
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