| 초록 |
In this study, bone-like microenvironmental structures which can be able to differentiate from BMSCs to osteogenic cells were fabricated for bone repair. And the effect of hydroxyapatite (HAp) as soluble factor was confirmed by adding various contents onto silk fibroin structure. Cell proliferation activity was measured via MTT assay, differentiation behavior was confirmed ALP activity assay and biological assay for osteocalcin, collagen I and RUNX2 was performed. As a result, various methods of analysis showed that the osteogenic differentiation value of 10% HAp onto silk fibroin was better than other groups. This study suggests that using bone-like microenvironmental structures with HAp become positive effect on osteogenesis of BMSC and osseous growth. This research was supported by NRF-2012M3A9C6050204, Bio-industry Technology Development Program (KMFAFF, 112007-05-2-SB010) and the Brain Korea 21 PLUS Project, NRF. |
