| 초록 |
Polymeric scaffolds should be porous enough to allow a high density of cells to be seeded, have sufficient mechanical stability and well-defined networks of interconnected pores. The regular, and high-interconnected macroporous poly(L-lactic acid)(PLLA) scaffold was fabricated from PLLA-dioxane-water ternary system with added PEG-PLLA diblock via thermally induced phase separation (TIPS). The porous morphology is determined by the final thermodynamic state and controlled by TIPS parameter. The pore size of scaffold in this system was obtained from 50mm to 300mm with the more aging time by coalescence process. PLLA scaffold is currently investigated for hard-tissue cell transplantation. MC3T3-El cell, osteoblast like cell line, is seeded and cultured in the produced PLLA scaffold. Cell Number is counted from BCA protein assay. The porous morphology and cell culture were characterized by scanning electron microscopy (SEM). |
