| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 봄 (04/07 ~ 04/08, 대전컨벤션센터) |
| 권호 |
36권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Immune Reaction of Purified and Nonpurified Alginate Microcapsules. |
| 초록 |
Alginate, a polysaccharide extracted from brown seaweed, remains the most widely used biomaterial for immobilizing cells to be transplanted. However, the main drawback is the immune rejection in vivo. To overcome this problem, we have developed using modified Korbutt’s method for alginate purification. After alginate microcapsules manufactured, NIH/3T3 fibroblast and RAW 264.7 macrophage cells were seeded in purified and non-purified alginate microcapsules, and then cell viability were analyzed by MTT assay. RT-PCR was performed to assess mRNA expression for inflammation cytokines such as TNF-α. Alginate microcapsules were implanted into a Wister rat, and the implanted alginate microcapsules were extracted after 1 and 2 weeks. Tissues surrounding implants were harvested and evaluated by histology through H&E and immunohistochemistry through ED-1 staining. This study demonstrates that purified alginate reduce inflammation reaction in vitro and in vivo |
| 저자 |
황지혜1, 김온유1, 김아람1, 윤건호2, 이동원1, 강길선1
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| 소속 |
1전북대, 2가톨릭대 |
| 키워드 |
Alginate; microcapsule; Inflammatory Reaction
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| E-Mail |
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