| 학회 |
한국화학공학회 |
| 학술대회 |
2008년 가을 (10/23 ~ 10/24, 부산 BEXCO) |
| 권호 |
14권 2호, p.2740 |
| 발표분야 |
생물화공 |
| 제목 |
Hydrogen production and metabolic flux analysis of metabolically engineered Escherichia coli strains |
| 초록 |
Escherichia coli can produce H2 from glucose via formate hydrogen lyase (FHL). In order to improve the H2 production rate and yield, metabolically engineered E. coli strains, which included pathway alterations in their H2 production and central carbon metabolism, were developed and characterized by batch experiments and metabolic flux analysis. Deletion of hycA, a negative regulator for FHL, resulted in 2-fold increase of FHL activity. Deletion of two up-take hydrogenases (1 (hya) and 2 (hyb)) increase H2 production yield from 1.20 to 1.48 mol/mol glucose. Deletion of lactate dehydrogenase (ldhA) and fumarate reductase (frdAB) further improved the H2 yield; 1.80 mol/mol glucose under high H2 pressure or 2.11 mol/mol glucose under reduced H2 pressure. Batch experiments at varying concentration of glucose (2.510 g/L) and yeast extract (0.3 or 3.0 g/L) were conducted for the strain containing all these genetic alternations, and carbon and energy balances were analyzed. The metabolic flux analysis revealed that deletion of ldhA and frdAB directed most of the carbons from glucose to the glycolytic pathway leading to H2 production by FHL, not to the pentose phosphate pathway. |
| 저자 |
김서형1, 설은희1, 장영아1, 오유관2, 박성훈1
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| 소속 |
1부산대, 2한국에너지기술(연) |
| 키워드 |
H2 production yield; Glucose fermentation; Metabolic engineering of Escherichia coli; Carbon and energy balance; Metabolic flux analysis
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| E-Mail |
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| 원문파일 |
초록 보기 |
