| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 봄 (04/07 ~ 04/08, 대전컨벤션센터) |
| 권호 |
36권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Redifferentiation of dedifferentiated chondrocyteson cell-derived extracellular matrix |
| 초록 |
The primary focus of this work is to evaluate naturally obtained cell-derived matrices (CDMs) as a physical cue in eliciting the redifferentiation of dedifferentiated chondrocytes under 2D environment. Different cell types-fibroblasts, preosteoblasts, chondrocytes- were cultured in monolayer for a week and then decellularized using a cocktail solution of detergents and enzymes. Each CDM was comprised of many components and their quantitative difference was significant. When dedifferentiated rat chondrocytes (P4) were cultured on each CDM, the gene expression of cartilage-specific markers indicated that chondrocytes grown on specific CDM carried much better sign of redifferentiation as compared to other groups. In addition, Immunofluorescene of type II collagen also exhibited the same outcome. In conclusion, CDMs may provide a favorable surface microenvironment for chondrogenic redifferentiation. |
| 저자 |
최동훈1, 차명화1, 한동근1, 김익환2, 박귀덕1
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| 소속 |
1한국과학기술(연), 2고려대 |
| 키워드 |
redifferentiation; chondrocytes; extracellular matrix
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| E-Mail |
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