| 학회 |
한국화학공학회 |
| 학술대회 |
2012년 가을 (10/24 ~ 10/26, 부산 BEXCO) |
| 권호 |
18권 2호, p.2756 |
| 발표분야 |
Nano/micro technology in cellular system symposium |
| 제목 |
Cell chip composed of nanopatterned array based on spectroelectrochemical technique |
| 초록 |
A cell chip was reported as a valuable tool for evaluating effects of various kinds of chemicals, drugs or nanomaterials on target cells with high accuracy. In this study, nanoscale film was developed on an artificial electrode surface to establish a cell-friendly environment which was effective for enhancing cell spreading, adhesion and proliferations. The film was further developed to three dimensional peptide nanopillar arrays which were more efficient than two dimensional peptide film in regard to various kinds of cellular functions. The electrochemical signals achieved from cells showed linear correlations with the cell viability which can be used as indicator for the toxicity assessment. Surface-enhanced Raman spectroscopy (SERS) was further applied as powerful tool to identify different cell lines, to discriminate alive- or dead- cells and to investigate toxicity of anticancer drugs. The combination of SERS and electrochemical technique was found to be excellent to study the alterations of intracellular composition of cells, as well as to investigate the internal redox properties of single cell. Proposed cell chip based on spectroelectrochemical technique can be used as an in vitro analysis tool in various kinds of biotechnology fields. |
| 저자 |
최정우 |
| 소속 |
서강대 |
| 키워드 |
세포독성; 나노 패턴; 전기화학; 표면증강라만분광법; 광전기화학
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