| 학회 | 한국공업화학회 |
| 학술대회 | 2017년 가을 (11/08 ~ 11/10, 부산 벡스코(BEXCO)) |
| 권호 | 21권 2호 |
| 발표분야 | 생물공학_포스터 |
| 제목 | Development of recombinant Pseudomonas putida for 3-HP production from glycerol - Disruption of 3-HP degradation pathway |
| 초록 | Biosynthesis 3-HP has been developed using recombinant microorganisms. Using Pseudomonas denitrificans for 3-HP production has several advantages, including: (i) aerobically organism; (ii) naturally producing B12 and (iii) 3-HP inducible systems is well-characterized. However, one of disadvantage is this strain only utilize gluconate as carbon source. In attempt to screen various hosts which has potential to produce 3-HP and also can utilize cheap carbon source (such as glucose), Pseudomonas putida could be a potential strain because of its characteristics. In this study, genes related to 3-HP degradation pathway were disrupted by using marker-less chromosomal gene deletion based on sacB selection system. Strain after removal of 3-HP catabolic genes was examined 3-HP degradation with growing cells and resting cells. As a result, this strain could produce 3-HP from glycerol by using plasmid overexpress genes in 3-HP pathway. |
| 저자 | NGUYEN THI TRINH |
| 소속 | UNIST |
| 키워드 | Pseudomonas putida; 3-Hydroxypropionic acid; Glycerol |
