| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 봄 (04/07 ~ 04/08, 대전컨벤션센터) |
| 권호 |
36권 1호 |
| 발표분야 |
대학원생 구두발표 |
| 제목 |
New cellular micropattern platforms for three-dimensional cell patterning and in situ metabolic detections |
| 초록 |
We fabricated micropatterned polymeric nanofibrous scaffold that are capable of micropatterning mammalian cells within biomimetic three-dimensional environment and detecting cellular metabolic products simultaneously. Photopatterning of poly(ethylene glycol) hydrogels on electrospun nanofibers created clearly defined hydrogel microwells incorporating polymeric nanofibers. The resultant micropatterned nanofibrous substrates were obtained as free-standing and bidirectionally porous sheets. When HepG2 cells were seeded onto resultant micropatterned scaffolds, cells selectively adhered within the fiber microdomains and formed spheroids. Size of spheroids could be controlled by varying the size of microwells. Due to increased surface area, antibody loading in nanofibrous substrates was greater than on planar substrates, which consequently yielded a higher fluorescence signal and faster reaction rate in immunoassays than conventional well-plate systems. |
| 저자 |
이현종, 고원건, 이열, 임태극
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| 소속 |
연세대 |
| 키워드 |
micropatterned scaffold; electrospinning; hydrogel microstructure; cell-based biosensor
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| E-Mail |
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