| 초록 |
To immobilize the effective and specific enzymes on nanomaterials such as nanoparticles, nanofiber and nanotube for stabilizing the activity of free enzymes has developed the various fields. In this results, Lysosomal enzymes isolated from hen`s egg white and Sacharomyces cerevisiae were directly immobilized on titanium (IV) oxide (TiO2) and the immobilization efficiency, antimicrobial activity using viable cell counts against Escherichia coli, and stability of lysosomal enzymes immobilized on TiO2 were evaluated. In addition, enhanced immobilization efficiency was shown in TiO2 pretreated with a divalent, positively charged ion, Ca2+, and the antimicrobial activity for E. coli increased as a function of increasing ratio of immobilized enzymes. Furthermore, the degradation of melanin for a week, we could determine the decrease of melanin in lysosomal enzymes immobilized on TiO2 after examining the effect of different pH of solvent on the immobilization of lysosomal enzymes extracted from S. cerevisiae and egg white.Therefore, our results suggest that the various activities of lysosomal enzymes immobilized on TiO2 may play an important role in applications for antimicrobial agents and cosmetics. |
